DYNAMIC LIGHT SCATTERING MAC

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DYNAMIC LIGHT SCATTERING MAC

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To schedule a training session please contact Ewa Folta-Stogniewįirst time users of the resource follow the booking and charging protocol please fill out the Biophysics Resource Usage Form from the box on the left **, and email the completely filled usage form, with email Subject: 'Biophysics Resource Usage,' to Ewa Folta-Stogniew. The Biophysical Resource at Keck Facility offers the Dynamic Light Scattering Detector (DynaPro, Wyatt Technology - formerly Protein Solutions) as an 'open access' instrument that can be used by trained users. The comparatively short time of an DLS measurement greatly facilitates carrying out the multiple studies that may be needed to determine the impact of protein concentration, ligands, pH. This calibration curve is provided by the manufacturer of the instrument based on the Rh measurement for well characterized proteins of known D and MW.Īlthough molecular weights can be determined also via mass spectrometry and analytical centrifugation, only light scattering and analytical centrifugation monitor the properties of macromolecules in solution and provide information about the oligomeric state of the protein. Molecular weight, MW, is calculated from the diffusion coefficient using a calibration plot of logD versus logM. If the sample is heterogeneous, it is possible (at least in principle) to obtain a distribution of Rh through mathematical manipulation of autocorrelation function. A simple way of representing heterogeneity is the polydispersity factor, which should be small (less than ~ 10%) for homogeneous sample. Additionally, the heterogeneity of the sample can be assessed by mathematical manipulations of the autocorrelation function. The hydrodynamic radius, Rh, can be then calculated from D using the Stokes-Einstein equation. Analysis of the correlation function decay as a function of short time intervals can be used to evaluate the translational diffusion coefficient, D. How rapidly the intensity fluctuates over time is represented by an autocorrelation function. The DLS experiment measures time fluctuations of light intensity caused by motions of macromolecules in solution. The measurement is faster than during static light scattering measurement and, most importantly, sample does not experience any dilution during measurement. Although this approach does not provide the precision possible with static light scattering detection, the measurement requires small amounts of protein (minimal amount for a protein of MW ~40 kDa is 25 microliters of a sample with ~0.5 mg/ml). We have recently expanded our light scattering service by implementing DYNAMIC LIGHT SCATTERING instrumentation.

Dynamic light scattering